OPTIMISATION OF AN IN VITRO PROPAGATION PROTOCOL FOR A VALUABLE LILY (Lilium spp.)

Abstract

Lily species have been used as ornamental plants for centuries. However, micropropagation of Lily in vitro depends on particular species. Therefore, in this study, a protocol for micro propagating Lily was optimized. The results indicated that in vitro type 1 Lily scales (near the basal stem) cultured on MS medium supplemented with 60 g/l saccharose, 0 g/l glucose, 0.5 mg/l BA, 0.1 mg/l NAA, 100 ml/l coconut water, 5.5 g/l agar and 1 g/l activated charcoal in full dark conditions is the best with the highest regeneration rate of bulbscale (3.68 bulblet/slice). In vitro Lily bulblets became healthy and bigger, formed roots in MS medium supplemented with 0.2 mg/l NAA.  In vitro Lily bulblets were found unsurvival and ungerminated without pre-cold treatment. The cold treatment time can vary between 4, 6, and 8 or 10 weeks, that did not affect the plant height and leaf numbers. The studies also found that the weight of bulblets significant affect plantlet height and leaf numbers.