TESTING THREE PROPOSED DNA REGIONS (matK, rbcL and ITS2)  FOR IDENTIFICATION OF CAMELLIA EUPHLEBIA  AND CAMELLIA CHRYSANTHA

Nguyen Van Viet; Pham Quang Trung; Do Quang Trung; Tran Viet Ha; Sounthone Douangmala

TESTING THREE PROPOSED DNA REGIONS (matK, rbcL and ITS2) FOR IDENTIFICATION OF CAMELLIA EUPHLEBIA AND CAMELLIA CHRYSANTHA

Authors

Nguyen Van Viet Vietnam National University of Forestry
Pham Quang Trung Vietnam National University of Forestry
Do Quang Trung Vietnam National University of Forestry
Tran Viet Ha Vietnam National University of Forestry
Sounthone Douangmala Bolykhamxay College of Agriculture and Forestry, Vientiane, Laos

Keywords:

Camellia chrysantha, Camellia euphlebia, DNA barcode, species identification, Yellow flower tea

Abstract

Camellia sp. is a yellow flower tea species that have high economic values and have been used as a nutritious beverage, medicine and an ornamental plant. The identification of these species are based on morphological characteristics and recently by molecular markers such as matK, ITS2, rbcL. This paper shows that the nucleotide sequenc of three DNA regions (matK, ITS2, rbcL) can be used to identify Camellia sp. collected from different areas in Vietnam (Quang Ninh and Bac Giang).The results also show that those three locations can be amplified by directional PCR. In addition, the nucleotide differences among those DNA regions of the two Camellia species ranged from 0% to 0.82% suggesting the Camellia chrysantha (Ba Che, Quang Ninh) is a derivative of Camellia euphlebia (Son Dong, Bac Giang).

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Published

15-05-2022

How to Cite

Van Viet, N., Quang Trung, P., Quang Trung, D., Viet Ha, T., & Douangmala, S. (2022). TESTING THREE PROPOSED DNA REGIONS (matK, rbcL and ITS2) FOR IDENTIFICATION OF CAMELLIA EUPHLEBIA AND CAMELLIA CHRYSANTHA. Journal of Forestry Science and Technology, (7), 018–024. Retrieved from http://journal.vnuf.edu.vn/en/article/view/771