MICROBIOLOGYCAL CHARACTERIZATION AND POTENTIAL APPLICATION OF INDIGENOUS B.METHYLOTROPHYCUS Ba1 IN HANDLING OF CANNA EDULIS. KER PROCESSING CRAFT VILLAGE WASTEWATER

Nguyen Nhu Ngoc; Nguyen Van Cach; Tran Lien Ha; Pham Thi Thuy Giang

Authors

Nguyen Nhu Ngoc Vietnam National University of Forestry
Nguyen Van Cach Hanoi University of Science and Technology
Tran Lien Ha Hanoi University of Science and Technology
Pham Thi Thuy Giang Hanoi University of Science and Technology

Keywords:

Activated sludge, aerobi, Bacillus methylotrophicus, selection, wastewater treatment

Abstract

Canna edulis. Ker starch processing wastewater from craft villages contains high organic concentration such as starch, cellulose, xylan, protein… and especially high starch concentration after the states of grinding, sedimentation and filtration. The concentration of COD and BOD₅, SS and coliform of wastewater are hundreds times higher than standards. To handle and recover organic pollutants of this wastewater, we focus on strains that are able to efficiently convert organic pollutants into biomass. The microbial biomass along with activated sludge – a kind of material can be applied to many other purposes are removed out by settling well while processing. From starch processing sludge and wastewater, strain of Bacillus methylotrophycus Ba₁ was selected with notable features: having the ability to use a variety of substrates (starch, cellulose, xylan, protein) with highest amylase activity (D/d = 12.5), COD removed efficiency (90% after 12 hours) and good sedimentation of activated sludge with SVI = 120.4. The results also showed that the optimal conditions for its biomass production: temperature 30^oC, initial pH 6.0 and inoculum of 7% (v/v). After 24 hours incubation at these conditions, the highest biomass was measured OD₆₀₀ of 2.91.